Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232306_b4ny GAAGGCAGGTCCCCAGTTGTTGGGCCAGCAGG(+ATATAT)GTACCTTCCTTCTTCTGCAGACTGCGATAGT NGG 10 8 17 8 24 0 100 5 10
    240606232304_09kn GTCACACTATCGCAGTCTGCAGAAGAAGGAAG(+ATATAT)GTACCCTGCTGGCCCAACAACTGGGGACCTG NGG 10 8 17 8 24 0 100 5 10
    240606232303_6t8b ACGCCTTTAGTCTCCGGATGTCTCAAATTATG(+ATATAT)GATCCTGAGCGCAGCCGTCTGCTCTTTCATG NGG 10 8 17 8 24 0 100 5 10
    240606232301_3s74 TTATGCATGAAAGAGCAGACGGCTGCGCTCAG(+ATATAT)GATCCATAATTTGAGACATCCGGAGACTAAA NGG 10 8 17 8 24 0 100 5 10
    240606232300_zk49 CCTGTGTCTCAGGGACGCCTCTACTTTCTTGG(+ATATAT)GATCCGGGACCGTTCTCAGAGGCACTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240606232257_4g3a TTCCGTCCCCAGTGCCTCTGAGAACGGTCCCG(+ATATAT)GATCCCAAGAAAGTAGAGGCGTCCCTGAGAC NGG 10 8 17 8 24 0 100 5 10
    240606232255_uhzw CAGCAAGATGCTCCTGATGCCCTTTGGTCCAG(+ATATAT)GATCCACTGCCTCCTTGTTCTAGAGGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606232254_opuv GATGTCTGAGCCTCTAGAACAAGGAGGCAGTG(+ATATAT)GATCCTGGACCAAAGGGCATCAGGAGCATCT NGG 10 8 17 8 24 0 100 5 10
    240606232252_yvz8 CCGCATGATGGCTGGGTCTGAAATCGCAAATG(+ATATAT)GATCCAGCCTGATGTCCTCGCAGTTGCACAT NGG 10 8 17 8 24 0 100 5 10
    240606232250_o6d3 CCCACATGTGCAACTGCGAGGACATCAGGCTG(+ATATAT)GATCCATTTGCGATTTCAGACCCAGCCATCA NGG 10 8 17 8 24 0 100 5 10