Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232151_q10w GCCTCTCCAGCCCCCGCTGGGCTGCAGCCAGG(+ATATAT)GTACCATCAACAGCCATGTTAAGGTTCTGAT NGG 10 8 17 8 24 0 100 5 10
    240606232150_0kl9 TTTTCATCAGAACCTTAACATGGCTGTTGATG(+ATATAT)GTACCCTGGCTGCAGCCCAGCGGGGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606232148_66v8 CTTCTCCATGGCTGCCAGGCCCCCCCAGGCAG(+ATATAT)GTACCTGCAGCGGCTGCCAGCCTGAGGGACT NGG 10 8 17 8 24 0 100 5 10
    240606232146_njuv ACTTCAGTCCCTCAGGCTGGCAGCCGCTGCAG(+ATATAT)GTACCTGCCTGGGGGGGCCTGGCAGCCATGG NGG 10 8 17 8 24 0 100 5 10
    240606232144_ld4o CAGCTTGGGCCAGCTGCTCTGAGCCTCCAAGG(+ATATAT)GATCCCCCGCCCCCTCCCCTGGAATGAAATC NGG 10 8 17 8 24 0 100 5 10
    240606232141_sxl3 GAAATGATTTCATTCCAGGGGAGGGGGCGGGG(+ATATAT)GATCCCTTGGAGGCTCAGAGCAGCTGGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606232139_7f15 CATCAGAGGAGAGTAAAGCAGGGACAACAGGG(+ATATAT)GTACCCAGCCTAGATCTTGTTGCTGAATCCA NGG 10 8 17 8 24 0 100 5 10
    240606232137_re8u ACCACTGGATTCAGCAACAAGATCTAGGCTGG(+ATATAT)GTACCCCTGTTGTCCCTGCTTTACTCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606232135_8949 TAGCAAACTCCCCAGTCCCCACAGCCACTCGG(+ATATAT)GTACCTCTTTTCCATAGACAGTTCTGATCAC NGG 10 8 17 8 24 0 100 5 10
    240606232133_xnnk CACACGTGATCAGAACTGTCTATGGAAAAGAG(+ATATAT)GTACCCGAGTGGCTGTGGGGACTGGGGAGTT NGG 10 8 17 8 24 0 100 5 10