Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232035_hfpw ATAATCTCTGTCCTCTTCTGAAATTGAAAATG(+ATATAT)GTACCCAACCCTCAGAGAGCGCAAGGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606232032_sguz TGTGGACAGTGGGCAGAAGCAGAGGCTCCCTG(+ATATAT)GATCCTGAACAAGGGCCCCAAAAGCAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606232031_j059 CTTTAGAACTTGCTTTTGGGGCCCTTGTTCAG(+ATATAT)GATCCAGGGAGCCTCTGCTTCTGCCCACTGT NGG 10 8 17 8 24 0 100 5 10
    240606232028_klc9 CGTGCCATCCCCATCAGGACCTCAGACCTGGG(+ATATAT)GTACCTGAACTCTGTCCCTCTGCTGCCGCCT NGG 10 8 17 8 24 0 100 5 10
    240606232026_foq4 GACAGAGGCGGCAGCAGAGGGACAGAGTTCAG(+ATATAT)GTACCCCAGGTCTGAGGTCCTGATGGGGATG NGG 10 8 17 8 24 0 100 5 10
    240606232024_smwt TCCTCACTCATTTTCAACGCTGACCCTGACAG(+ATATAT)GATCCACAGACTTCTCCAGGACCTTCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606232022_k7wj AGGGTCTCTGGAAGGTCCTGGAGAAGTCTGTG(+ATATAT)GATCCTGTCAGGGTCAGCGTTGAAAATGAGT NGG 10 8 17 8 24 0 100 5 10
    240606232020_nelo TCCCCCAGGGGAGAGGCCCCTTTACCTCAGTG(+ATATAT)GATCCTGTTAAGGAGTGCTTCCAGCCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240606232019_16pd CCCAGGCTGAGGCTGGAAGCACTCCTTAACAG(+ATATAT)GATCCACTGAGGTAAAGGGGCCTCTCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606232017_b9br CCTACAGTGTTACTTCCTAGGGGCTACGCAGG(+ATATAT)GATCCGTTTCTCTCCACTGTGCTATTCACAA NGG 10 8 17 8 24 0 100 5 10