Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606231914_viy1 CACACACACACACAGTGCAGACCCCAGAAAGG(+ATATAT)GATCCCCAGTCTGGCCCCCAGCCTGGATAGA NGG 10 8 17 8 24 0 100 5 10
    240606231912_z67j TGACTTTTCATGGGCGTGCTCTAGGAATCCTG(+ATATAT)GATCCCACTAAACTTCCATGGCTGGTTGGGC NGG 10 8 17 8 24 0 100 5 10
    240606231910_g53i CCCGTGCCCAACCAGCCATGGAAGTTTAGTGG(+ATATAT)GATCCAGGATTCCTAGAGCACGCCCATGAAA NGG 10 8 17 8 24 0 100 5 10
    240606231906_m1hc GCCCAGCCTGACTGCTGTGCTCTGGGCCTGGG(+ATATAT)GATCCATGAGGTTCTTATGTAGAAGCTGAGG NGG 10 8 17 8 24 0 100 5 10
    240606231905_xnlr AGAGACCTCAGCTTCTACATAAGAACCTCATG(+ATATAT)GATCCCCAGGCCCAGAGCACAGCAGTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606231903_wace CCTCTTCCCTCTGCCCAGCACCTAAGAGCAGG(+ATATAT)GATCCCACGGCGGGGAGATGAGCAGGGGTGC NGG 10 8 17 8 24 0 100 5 10
    240606231900_qgrn AGACCGCACCCCTGCTCATCTCCCCGCCGTGG(+ATATAT)GATCCCTGCTCTTAGGTGCTGGGCAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606231858_p2pe TCCTAAGGGAGCCAGATGTCAAGTCCAGGGTG(+ATATAT)GTACCCGATAACAATCTCCTGGGCTTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240606231857_i3qq TCAGGACCTGAAGCCCAGGAGATTGTTATCGG(+ATATAT)GTACCACCCTGGACTTGACATCTGGCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606231854_n4k3 GTCAACTAAGGCGGCTATTGTTTGTGCCTCAG(+ATATAT)GATCCTAAGGGAGCCAGATGTCAAGTCCAGG NGG 10 8 17 8 24 0 100 5 10