Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606231956_36cg AGCACTTGTGTGCACAGACCCAGGTAACAACG(+ATATAT)GTACCAAGCAAGCTGGAACTCATACAGTAAT NGG 10 8 17 8 24 0 100 5 10
    240606231954_iu5v ACCCTATGCCTAAGACCTCTTTAGTTTGGGAG(+ATATAT)GATCCCCCTCACTCTCCTGGAGCAAGTATAC NGG 10 8 17 8 24 0 100 5 10
    240606231951_1v2f CACTGGTATACTTGCTCCAGGAGAGTGAGGGG(+ATATAT)GATCCTCCCAAACTAAAGAGGTCTTAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606231950_435s AAGCCAACAAACTGAGAATTTGCCCCTGTAAG(+ATATAT)GATCCATGTGGTAGATACTAGCTGCTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606231948_jkqk TTGGCCAAAGAGCAGCTAGTATCTACCACATG(+ATATAT)GATCCTTACAGGGGCAAATTCTCAGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606231946_bqg7 TTAAAATCTAATACCTGTAGCACAAAGTTGTG(+ATATAT)GATCCATATAGCAGAAATTGGCTGCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606231944_sw44 GGGGTCAAAGGGCAGCCAATTTCTGCTATATG(+ATATAT)GATCCACAACTTTGTGCTACAGGTATTAGAT NGG 10 8 17 8 24 0 100 5 10
    240606231942_pzp9 CCACATGGTGGTTCACAACCATCTGTAATGGG(+ATATAT)GATCCATATACATAAATAAACCTTTTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606231940_tb8p AATATTTTTTAAAAGGTTTATTTATGTATATG(+ATATAT)GATCCCCATTACAGATGGTTGTGAACCACCA NGG 10 8 17 8 24 0 100 5 10
    240606231938_1v11 TTAAAATCTAATACCTGTAGCACAAAGTTGTG(+ATATAT)GATCCATATAGCAGAAATTGGCTGCCCTTTG NGG 10 8 17 8 24 0 100 5 10