Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606231851_h9r6 ACCACCCTGGACTTGACATCTGGCTCCCTTAG(+ATATAT)GATCCTGAGGCACAAACAATAGCCGCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240606231850_pubg TCCAGCTTCTGCCAAGCTCCCCTAAGGAATTG(+ATATAT)GATCCAAAGTAAAATGGCCCATAGCTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606231848_qrpo TGACCAGGAGAGCTATGGGCCATTTTACTTTG(+ATATAT)GATCCAATTCCTTAGGGGAGCTTGGCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606231845_3vpf GAGGAGGTGTAGACTGGGAACTAGTGCCTGGG(+ATATAT)GTACCCCATCTTGAGGTCAGCAGCAAGTGGG NGG 10 8 17 8 24 0 100 5 10
    240606231843_l17s GTGGCCCCACTTGCTGCTGACCTCAAGATGGG(+ATATAT)GTACCCCAGGCACTAGTTCCCAGTCTACACC NGG 10 8 17 8 24 0 100 5 10
    240606231841_89s5 GCAAGCAAAAGGGAGGACTCCCAGAGCTTTGG(+ATATAT)GATCCAGTTGTGCCTCCCGGCTGACTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606231839_3pus AGCGAAGGCCAGTCAGCCGGGAGGCACAACTG(+ATATAT)GATCCCAAAGCTCTGGGAGTCCTCCCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240606231837_tgqy AGACCTGACTCAGATGCCACTCTGACCCCAAG(+ATATAT)GTACCCTGCCCTTCGTGTGTTTTAACAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606231835_fii6 TTATTGGCTTGTTAAAACACACGAAGGGCAGG(+ATATAT)GTACCTTGGGGTCAGAGTGGCATCTGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606231832_9kd1 TCCTCTGAGATCCCCACAAGTTCCTTTCTCGG(+ATATAT)GATCCTTTCACCCCTACCTCTTCCTGCCTGT NGG 10 8 17 8 24 0 100 5 10