Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606231732_rh98 GCATTAGTCCCGCCTCCTCCATCTTTCATGTG(+ATATAT)GTACCCAGGTAAATGTGGACATAGTGTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606231730_fqrz ATCAAGCAATCATCCCAAAAGGTAGCTCTGTG(+ATATAT)GTACCTCTGTTGACCCTGGCTGTGACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606231728_jm17 GACAGCCCAGGTCACAGCCAGGGTCAACAGAG(+ATATAT)GTACCACAGAGCTACCTTTTGGGATGATTGC NGG 10 8 17 8 24 0 100 5 10
    240606231727_w6ak TTCTCTTATCTCTGTTTCAAATCTCCCTGTAG(+ATATAT)GATCCTGAGGTTACAAACATGTGCCATAGCA NGG 10 8 17 8 24 0 100 5 10
    240606231724_yw21 CCAGATGCTATGGCACATGTTTGTAACCTCAG(+ATATAT)GATCCTACAGGGAGATTTGAAACAGAGATAA NGG 10 8 17 8 24 0 100 5 10
    240606231722_7k4l GATCTATCTTGTTATGCAGCCAGAATGGCCTG(+ATATAT)GATCCCCCTCCTGTGTTTTCAGGTGTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606231719_05s0 CAAAAGAGCAACACCTGAAAACACAGGAGGGG(+ATATAT)GATCCAGGCCATTCTGGCTGCATAACAAGAT NGG 10 8 17 8 24 0 100 5 10
    240606231717_kdb6 CCCTCCCATAGCAGGCATGCTGACACATCCTG(+ATATAT)GTACCCTGCCGTACTAACTGCTCTGCTGCCT NGG 10 8 17 8 24 0 100 5 10
    240606231714_ijip CGTTGAGGCAGCAGAGCAGTTAGTACGGCAGG(+ATATAT)GTACCAGGATGTGTCAGCATGCCTGCTATGG NGG 10 8 17 8 24 0 100 5 10
    240606231711_p2t1 CACCTTGCATATGTGAGGCCTTTGCCACCCAG(+ATATAT)GATCCTCTGACATCTGGGTGATTTCAGATGC NGG 10 8 17 8 24 0 100 5 10