Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606231611_grx8 GAGAGAGACAGAGAGAGAGAATGTGTCTGCAG(+ATATAT)GTACCAGGGAGGCACAAGAGGATGTCAGACC NGG 10 8 17 8 24 0 100 5 10
    240606231609_6mwr GTGTGCACTGCTTGTATGCGTGTGGATGTGTG(+ATATAT)GATCCCACCTGCCGCCTCCCCATCTGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240606231607_24lm ACACCCAGAGCAGATGGGGAGGCGGCAGGTGG(+ATATAT)GATCCACACATCCACACGCATACAAGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240606231605_hboi TCTGGGTGGGCGAGATACCCAATGCCAGCCAG(+ATATAT)GTACCAGCGCCCCAGGAGCATGAAATGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606231604_7z43 GTGGCCTGGCATTTCATGCTCCTGGGGCGCTG(+ATATAT)GTACCTGGCTGGCATTGGGTATCTCGCCCAC NGG 10 8 17 8 24 0 100 5 10
    240606231602_9nla GCTGTCTTCAGACACACCAGAACGGGGCATTG(+ATATAT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606231600_8tsb ACCACATGGTGGCTCACAACCATCTGTAATGG(+ATATAT)GATCCAATGCCCCGTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606231558_vbxu GTCCCCACTCCCCCGACCCAGCTCCACGGGAG(+ATATAT)GATCCAGGAGGCAACCCCAGCCCAGCTGGAG NGG 10 8 17 8 24 0 100 5 10
    240606231556_65fc GATGTCTCCAGCTGGGCTGGGGTTGCCTCCTG(+ATATAT)GATCCTCCCGTGGAGCTGGGTCGGGGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240606231554_3ms0 GCTGAACCCATCAGACGGGTCCGACTAGCCGG(+ATATAT)GATCCGAGGCCTTGGCCCAGGAGATAGGCAT NGG 10 8 17 8 24 0 100 5 10