Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606231454_lw1z TTTAGTGTATGTGTGTATCTAGGCTCACACGG(+ATATAT)GTACCTATGTGTAAACATGTTTGTCTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606231453_0645 AGACTTAAGAGTAGGAAAGGGACTGGTACATG(+ATATAT)GTACCTGCCAGCCCAGCCTAGCACGAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240606231451_289v AAAAAACTCTTCGTGCTAGGCTGGGCTGGCAG(+ATATAT)GTACCATGTACCAGTCCCTTTCCTACTCTTA NGG 10 8 17 8 24 0 100 5 10
    240606231448_lflt GCTGAGAATCACAGATTTAGCAGCTCCTAGGG(+ATATAT)GTACCCACCACATTGAGCTATGCTGAATTAA NGG 10 8 17 8 24 0 100 5 10
    240606231446_h70d AAATATTAATTCAGCATAGCTCAATGTGGTGG(+ATATAT)GTACCCCTAGGAGCTGCTAAATCTGTGATTC NGG 10 8 17 8 24 0 100 5 10
    240606231444_o4k1 TCCCCAGGCCCTTGATAACTTTTCCCCTTCAG(+ATATAT)GATCCCACTCATAGTAGATTTATTTTAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606231442_clcq AATCCTACTTAAAATAAATCTACTATGAGTGG(+ATATAT)GATCCTGAAGGGGAAAAGTTATCAAGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606231440_nd8p CTTGTACTTCTGTTACTCTAGAACCACTCCTG(+ATATAT)GTACCAACTTCTGTATTAGTCAGGGTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606231438_noyj ACTCTAGAGAACCCTGACTAATACAGAAGTTG(+ATATAT)GTACCAGGAGTGGTTCTAGAGTAACAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240606231437_zj5g TTCTTTAAAGTTCTGGAGAATGGGTGAAGCTG(+ATATAT)GTACCTTGCAGAGAAGGGACACAAGGAAGAT NGG 10 8 17 8 24 0 100 5 10