Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606231435_039k GTGGCATCTTCCTTGTGTCCCTTCTCTGCAAG(+ATATAT)GTACCAGCTTCACCCATTCTCCAGAACTTTA NGG 10 8 17 8 24 0 100 5 10
    240606231434_r835 AAAAACTTCAGAGTTGCAGACAGGACAGTCAG(+ATATAT)GTACCCCATGGGAGTTCCTAGGGCCTGCGTA NGG 10 8 17 8 24 0 100 5 10
    240606231432_k0nm ATCCATACGCAGGCCCTAGGAACTCCCATGGG(+ATATAT)GTACCTGACTGTCCTGTCTGCAACTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606231430_2snj ATGCGCTCTGCACTGCAGCCCCATCTATTCAG(+ATATAT)GATCCTGCCACCCATGAGGAGATGCCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606231428_ga4p TCCCGTGCTGGGCATCTCCTCATGGGTGGCAG(+ATATAT)GATCCTGAATAGATGGGGCTGCAGTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606231427_8apw GCTGCAGCCCCTGCAGCCCCAACCGCAAGCTG(+ATATAT)GATCCGGGATCTGCACATCAGCAGCCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240606231424_t3e4 GGGCTCCAAAGGCTGCTGATGTGCAGATCCCG(+ATATAT)GATCCAGCTTGCGGTTGGGGCTGCAGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606231423_zyia ACACTAAATAAAACCAAGCCAATAGTCTTGTG(+ATATAT)GATCCCTGAATATTTCTCATTCCACACTTCA NGG 10 8 17 8 24 0 100 5 10
    240606231421_fs1i TAAGATGAAGTGTGGAATGAGAAATATTCAGG(+ATATAT)GATCCACAAGACTATTGGCTTGGTTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240606231419_rbzz CTTGCCTGTCTGGTCAGCAGTATTGCACACAG(+ATATAT)GATCCACAGATGACAGTTGATGGCTCTCCCC NGG 10 8 17 8 24 0 100 5 10