Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606231318_46v3 GCACAGACCTAGGGATGCACTCCCTGCCTTTG(+ATATAT)GATCCCCACTCTACCATTTGCTAATTAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606231315_xqsv AAGGTCAACTAATTAGCAAATGGTAGAGTGGG(+ATATAT)GATCCAAAGGCAGGGAGTGCATCCCTAGGTC NGG 10 8 17 8 24 0 100 5 10
    240606231313_m6tj TCAAATCCAAAGCCGGCCTGTGTGATGTAGTG(+ATATAT)GTACCTCTTTCCATCTCAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606231312_ltke GACTTTTTTTTTTTTTTTTGAGATGGAAAGAG(+ATATAT)GTACCACTACATCACACAGGCCGGCTTTGGA NGG 10 8 17 8 24 0 100 5 10
    240606231310_u6en GAGGAGAGAAGACACTGGGGGCCTGGAGCTGG(+ATATAT)GTACCAGGGCTACTACTGCCTTCCACTGTGA NGG 10 8 17 8 24 0 100 5 10
    240606231307_pi2m TGGTCTCACAGTGGAAGGCAGTAGTAGCCCTG(+ATATAT)GTACCCAGCTCCAGGCCCCCAGTGTCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240606231305_1zxa GAGGCCAGCCTGGGTTAGAGTGACATGTAGTG(+ATATAT)GATCCTCATTATGTGTTCATGTTAGAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606231304_snjs TTGAGCTTTTTCTAACATGAACACATAATGAG(+ATATAT)GATCCACTACATGTCACTCTAACCCAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606231301_9sja CCATACCCTAAATGTATTCTATACTATACCTG(+ATATAT)GTACCTCAGGGGGAAGGGATGCCAGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240606231259_59na GTGGACCCATGCTGGCATCCCTTCCCCCTGAG(+ATATAT)GTACCAGGTATAGTATAGAATACATTTAGGG NGG 10 8 17 8 24 0 100 5 10