Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606231156_w35m GCCTTCATCCCTCACCTCAGAGCGAAACACTG(+ATATAT)GATCCAGCCCCTTTGTCACCATCACTTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606231154_ml52 GGCCTAGAAAAGTGATGGTGACAAAGGGGCTG(+ATATAT)GATCCAGTGTTTCGCTCTGAGGTGAGGGATG NGG 10 8 17 8 24 0 100 5 10
    240606231152_z84f CTTCATATAGCCCAGGCTAGACCCAAACTCAG(+ATATAT)GATCCTCCTGCCTCCACCTCCAGAGTGTTGA NGG 10 8 17 8 24 0 100 5 10
    240606231150_bmq1 TAATCTCAACACTCTGGAGGTGGAGGCAGGAG(+ATATAT)GATCCTGAGTTTGGGTCTAGCCTGGGCTATA NGG 10 8 17 8 24 0 100 5 10
    240606231148_p68q GTTTTGATTCTCATGAGTAAAGAAGAAACAAG(+ATATAT)GTACCCACCCCTGGATGCTGGACGAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606231146_k68q AGGGGCCTCTCTCGTCCAGCATCCAGGGGTGG(+ATATAT)GTACCTTGTTTCTTCTTTACTCATGAGAATC NGG 10 8 17 8 24 0 100 5 10
    240606231145_57n2 TCCCATTAAGGAAAATGTGAAAATTGCATATG(+ATATAT)GATCCACTCTCACCATAAAGGTGAGAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606231143_elov CCTGCCTTCTTCTCACCTTTATGGTGAGAGTG(+ATATAT)GATCCATATGCAATTTTCACATTTTCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240606231142_evl0 AGTGTCTGGGTTTGCTGTCTGCAAGTGGAATG(+ATATAT)GATCCCAAGGTGGGCCAGTGTCTTGATGGCC NGG 10 8 17 8 24 0 100 5 10
    240606231139_nbz6 GGAAAGGCCATCAAGACACTGGCCCACCTTGG(+ATATAT)GATCCATTCCACTTGCAGACAGCAAACCCAG NGG 10 8 17 8 24 0 100 5 10