Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606231236_au7g AGACAATTCCTGTTCAGAGACAACAGGGGCCG(+ATATAT)GTACCACTGGGAGATGGCAAAAGTTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606231233_4kpc GGGTGGGAAGAAACTTTTGCCATCTCCCAGTG(+ATATAT)GTACCGGCCCCTGTTGTCTCTGAACAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240606231232_hst3 GCAAGAGTCGGTTCCCTCCTTCTACTGTGTAG(+ATATAT)GTACCAGTGATTAAACAGGCCGTTGAGCTCG NGG 10 8 17 8 24 0 100 5 10
    240606231230_ki2b GCCTCCGAGCTCAACGGCCTGTTTAATCACTG(+ATATAT)GTACCTACACAGTAGAAGGAGGGAACCGACT NGG 10 8 17 8 24 0 100 5 10
    240606231228_rwwo TATCACCACCCTGTGCAGTCAGAGAGGTCTGG(+ATATAT)GATCCGGTCTCTACTCTGCATCTGGTGATCC NGG 10 8 17 8 24 0 100 5 10
    240606231226_zrq4 CAGATGGATCACCAGATGCAGAGTAGAGACCG(+ATATAT)GATCCCAGACCTCTCTGACTGCACAGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240606231224_8epg GGAACCCCTTGCACTGTGAAGCTAGAGCGTGG(+ATATAT)GTACCTAACTCACCGTTGTCCCAGAATGAGG NGG 10 8 17 8 24 0 100 5 10
    240606231222_q7wg CCCCGCCTCATTCTGGGACAACGGTGAGTTAG(+ATATAT)GTACCCACGCTCTAGCTTCACAGTGCAAGGG NGG 10 8 17 8 24 0 100 5 10
    240606231219_yed4 TCTGCGTGGGAGAACTTGGTGTCTCACCCTGG(+ATATAT)GTACCCATTCCTGGTGTATGGAAGCACCTCC NGG 10 8 17 8 24 0 100 5 10
    240606231217_21ak GTGAAGGAGGTGCTTCCATACACCAGGAATGG(+ATATAT)GTACCCAGGGTGAGACACCAAGTTCTCCCAC NGG 10 8 17 8 24 0 100 5 10