Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606231935_jyqu GGGGTCAAAGGGCAGCCAATTTCTGCTATATG(+ATATAT)GATCCACAACTTTGTGCTACAGGTATTAGAT NGG 10 8 17 8 24 0 100 5 10
    240606231933_tw18 CTAGGGAGCCATCTGGGATGTTGGCATGTTGG(+ATATAT)GTACCTGTCCTGTGACACTTGATTGTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606231931_aghs CCACCCAAGCACAATCAAGTGTCACAGGACAG(+ATATAT)GTACCCAACATGCCAACATCCCAGATGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606231929_dvv8 GGAGAGTCACTGGCCTCTCCCGTTGGTTACAG(+ATATAT)GTACCCTTATACCCTGGATGTTAGCGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240606231927_bi31 ACAACTACAACGCTAACATCCAGGGTATAAGG(+ATATAT)GTACCTGTAACCAACGGGAGAGGCCAGTGAC NGG 10 8 17 8 24 0 100 5 10
    240606231925_1a5y CTTGAAAAGCTGGCAGCACTGCAGCCTCCCTG(+ATATAT)GTACCATTGGTCTGACAGACACAGTTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606231922_ya3o GACTATCTGGAACTGTGTCTGTCAGACCAATG(+ATATAT)GTACCAGGGAGGCTGCAGTGCTGCCAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606231920_k27x CGGAGGATGAAGCCCATCCCTTGCAGATGGGG(+ATATAT)GTACCCATGCTTTGATGATGTTCACAGAGGG NGG 10 8 17 8 24 0 100 5 10
    240606231918_0qfk AGAAGCCCTCTGTGAACATCATCAAAGCATGG(+ATATAT)GTACCCCCATCTGCAAGGGATGGGCTTCATC NGG 10 8 17 8 24 0 100 5 10
    240606231916_ry3g TCGTGTCTATCCAGGCTGGGGGCCAGACTGGG(+ATATAT)GATCCCTTTCTGGGGTCTGCACTGTGTGTGT NGG 10 8 17 8 24 0 100 5 10