Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232113_w1dy TCCTCATGTAGCTCACAAGGGCTGGTGTGGAG(+ATATAT)GATCCGCCTTCTTGCTGGAGAACCCAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606232111_nlmw TAGGGGAGGCTGGGTTCTCCAGCAAGAAGGCG(+ATATAT)GATCCTCCACACCAGCCCTTGTGAGCTACAT NGG 10 8 17 8 24 0 100 5 10
    240606232109_x6em GGCAGAGATGGCTCCATGTTTTACAAACTATG(+ATATAT)GATCCCTGGGCAGAGTTCAGGTGTCCCTGTA NGG 10 8 17 8 24 0 100 5 10
    240606232106_efhr AAGGATACAGGGACACCTGAACTCTGCCCAGG(+ATATAT)GATCCATAGTTTGTAAAACATGGAGCCATCT NGG 10 8 17 8 24 0 100 5 10
    240606232104_p5ud GAGGCATGTCAGCTACCAGTTTCTCCACAGAG(+ATATAT)GATCCAAGAAGTACATCAAAGATGGTCTACC NGG 10 8 17 8 24 0 100 5 10
    240606232103_c2cu GAGATGGTAGACCATCTTTGATGTACTTCTTG(+ATATAT)GATCCTCTGTGGAGAAACTGGTAGCTGACAT NGG 10 8 17 8 24 0 100 5 10
    240606232101_bvzu CAGTGAGTGCCAGCGGTGCTGGAGCTAGAGCG(+ATATAT)GATCCTGAAGGCAGTGACATTGGGACAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606232100_grdt GACCCTGACTGTCCCAATGTCACTGCCTTCAG(+ATATAT)GATCCGCTCTAGCTCCAGCACCGCTGGCACT NGG 10 8 17 8 24 0 100 5 10
    240606232057_kpr5 TAAGAAGAGTCTCCCCTGAAGTGTTTGTGGTG(+ATATAT)GTACCTGTGTGCAGAAGGGTGTGTCGCCACT NGG 10 8 17 8 24 0 100 5 10
    240606232056_2iw6 TGCCAAGTGGCGACACACCCTTCTGCACACAG(+ATATAT)GTACCACCACAAACACTTCAGGGGAGACTCT NGG 10 8 17 8 24 0 100 5 10