Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232228_bwwk CCTGAGCCTCCCTCGGGCACCTGTTGCTTCTG(+ATATAT)GATCCGAATAATCCAGGAGGCTCCCCACCCT NGG 10 8 17 8 24 0 100 5 10
    240606232226_frc2 GCCACAGGGTGGGGAGCCTCCTGGATTATTCG(+ATATAT)GATCCAGAAGCAACAGGTGCCCGAGGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240606232225_ulc5 GCCACACACATACCGAGACTCTGTGAATGATG(+ATATAT)GTACCACTCTACCTAGGACAGGAGAGACCGA NGG 10 8 17 8 24 0 100 5 10
    240606232223_3rwp CTTTGTCGGTCTCTCCTGTCCTAGGTAGAGTG(+ATATAT)GTACCATCATTCACAGAGTCTCGGTATGTGT NGG 10 8 17 8 24 0 100 5 10
    240606232222_k2u9 CCCCGCCTTCAGGAAGACTTCCAGGACTGACG(+ATATAT)GTACCCAGTCTCGGCGCCCTCCTCCCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606232220_mcmc AAGGGCTCCTGGGAGGAGGGCGCCGAGACTGG(+ATATAT)GTACCGTCAGTCCTGGAAGTCTTCCTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606232218_e0sw CTCAACCAATGAAAAGTCAAAAGTCCTCCTAG(+ATATAT)GATCCTGTGTAGCATTCCTGGGCCTGTTCCA NGG 10 8 17 8 24 0 100 5 10
    240606232216_x4sw GAGAATGGAACAGGCCCAGGAATGCTACACAG(+ATATAT)GATCCTAGGAGGACTTTTGACTTTTCATTGG NGG 10 8 17 8 24 0 100 5 10
    240606232214_gg52 TGCCGGCTTTCAGCTTGGCTGGGCTGGGCTGG(+ATATAT)GATCCTGAACTGTATCCATTATCCCTGGGTG NGG 10 8 17 8 24 0 100 5 10
    240606232213_shkq TTTCGCACCCAGGGATAATGGATACAGTTCAG(+ATATAT)GATCCCAGCCCAGCCCAGCCAAGCTGAAAGC NGG 10 8 17 8 24 0 100 5 10