Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232132_fugg TCTGGCATTTCATGTTACTGCAGGCCTTTGAG(+ATATAT)GTACCTCAATGTGTAAGATATTTCTGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606232130_2osc TTAAGCCCCACAGAAATATCTTACACATTGAG(+ATATAT)GTACCTCAAAGGCCTGCAGTAACATGAAATG NGG 10 8 17 8 24 0 100 5 10
    240606232128_67s8 ATACTGAAACAGACTTCCAACAGGAAGGCACG(+ATATAT)GTACCTGAGAGCAAGTGTTTCTGAATAGAAC NGG 10 8 17 8 24 0 100 5 10
    240606232127_4fgi GTCCAGTTCTATTCAGAAACACTTGCTCTCAG(+ATATAT)GTACCGTGCCTTCCTGTTGGAAGTCTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240606232124_4gxb TAAATGACAGTGTGTAACTATCTGTAACTGGG(+ATATAT)GATCCCATGCCCTTTTCTGACCTTCAAGGTC NGG 10 8 17 8 24 0 100 5 10
    240606232122_cxtd TTGGTGACCTTGAAGGTCAGAAAAGGGCATGG(+ATATAT)GATCCCCAGTTACAGATAGTTACACACTGTC NGG 10 8 17 8 24 0 100 5 10
    240606232120_68u6 GCCGTTAGACTGCCTGCCTTTCAGAGGTCTGG(+ATATAT)GATCCTGGCTCCCCACCCCCAAGTGCGAGAC NGG 10 8 17 8 24 0 100 5 10
    240606232118_szm3 AAATGGTCTCGCACTTGGGGGTGGGGAGCCAG(+ATATAT)GATCCCAGACCTCTGAAAGGCAGGCAGTCTA NGG 10 8 17 8 24 0 100 5 10
    240606232116_4668 TTAGGAACAAGGCAGAGAGCACAGTCGCTCTG(+ATATAT)GATCCACCCTAAAAAGCCTGCTGACGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240606232114_8p7c TGTCCCTCCACGTCAGCAGGCTTTTTAGGGTG(+ATATAT)GATCCAGAGCGACTGTGCTCTCTGCCTTGTT NGG 10 8 17 8 24 0 100 5 10