Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232406_2819 GGCCGCCTCCTCGGTACAGCCAGCATTTGCTG(+ATATAT)GTACCCATTGTATCCCTTCTGCCCACCTGTC NGG 10 8 17 8 24 0 100 5 10
    240606232404_ewe7 TCTTGGACAGGTGGGCAGAAGGGATACAATGG(+ATATAT)GTACCAGCAAATGCTGGCTGTACCGAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606232401_hwsi ACTGAACCTGGAGCTGCCCAGCAGGCTTCCGG(+ATATAT)GATCCTCCAGGGTCTGCTTCCACAGAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606232359_bonx GACTCCTGGCTCTGTGGAAGCAGACCCTGGAG(+ATATAT)GATCCCGGAAGCCTGCTGGGCAGCTCCAGGT NGG 10 8 17 8 24 0 100 5 10
    240606232357_833o GAGTCAGTTCTTGAGCCCAGCTTTCCAGTGAG(+ATATAT)GATCCGCACCTGCGGGATGTGTGTCCTATTC NGG 10 8 17 8 24 0 100 5 10
    240606232356_h2g7 CACATGAATAGGACACACATCCCGCAGGTGCG(+ATATAT)GATCCTCACTGGAAAGCTGGGCTCAAGAACT NGG 10 8 17 8 24 0 100 5 10
    240606232353_zdie AATAATGCTCACTTCCCAGTCTGTGGCCACGG(+ATATAT)GATCCCATGTGTCTTTTTGCTTGTGGCAGAT NGG 10 8 17 8 24 0 100 5 10
    240606232350_p4at AAGAAATCTGCCACAAGCAAAAAGACACATGG(+ATATAT)GATCCCGTGGCCACAGACTGGGAAGTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606232349_x32i AGGGCAGGACCTGGTCCATCTCTGCGGGACTG(+ATATAT)GTACCAACCAATAAGGCAGAGGGGGTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240606232347_yaok GGCGAGCTCAACCCCCTCTGCCTTATTGGTTG(+ATATAT)GTACCAGTCCCGCAGAGATGGACCAGGTCCT NGG 10 8 17 8 24 0 100 5 10