Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232602_en4v CAGGCCAGGACCCACACAACTGATGGGGGCAG(+ATATAT)GATCCCCAGTGATGGGTCCAGAGTGGGAATC NGG 10 8 17 8 24 0 100 5 10
    240606232559_zm13 GACTTGATTCCCACTCTGGACCCATCACTGGG(+ATATAT)GATCCTGCCCCCATCAGTTGTGTGGGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606232557_fmzj GGTCTCCCGAGAGCATGCCGAGTGTCCTGAGG(+ATATAT)GATCCTCGGCTAGCCCCCATCTTCTACTTGA NGG 10 8 17 8 24 0 100 5 10
    240606232555_ekru AGCCTTCAAGTAGAAGATGGGGGCTAGCCGAG(+ATATAT)GATCCCTCAGGACACTCGGCATGCTCTCGGG NGG 10 8 17 8 24 0 100 5 10
    240606232553_pv33 GGAGGGAACCTTGGCAGCACTGGGCCTGTAGG(+ATATAT)GATCCAGAGTTCTCAGGCAATAGAAGTGATC NGG 10 8 17 8 24 0 100 5 10
    240606232552_7462 ACCCAGATCACTTCTATTGCCTGAGAACTCTG(+ATATAT)GATCCCTACAGGCCCAGTGCTGCCAAGGTTC NGG 10 8 17 8 24 0 100 5 10
    240606232550_xp06 GAGGGTGGTCTGTCGCATCACCACATTAGTGG(+ATATAT)GTACCTGACAGTTACCTCATTGGCAGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240606232548_jx2s CTCACCTTGGCTGCCAATGAGGTAACTGTCAG(+ATATAT)GTACCCACTAATGTGGTGATGCGACAGACCA NGG 10 8 17 8 24 0 100 5 10
    240606232546_93gv CTGCCCCTCTGACTCCTGCCCAGAAGTGGTGG(+ATATAT)GTACCCCCCCACCCCGTGCCATTCATTGTCC NGG 10 8 17 8 24 0 100 5 10
    240606232541_mb3k GGGTGGGACAATGAATGGCACGGGGTGGGGGG(+ATATAT)GTACCCACCACTTCTGGGCAGGAGTCAGAGG NGG 10 8 17 8 24 0 100 5 10