Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232623_p2ws TAAGTGAGACAAGAGCCCAGCCCAACCTCAGG(+ATATAT)GATCCAACAGTCCACGGCCATCTCCTTAGCT NGG 10 8 17 8 24 0 100 5 10
    240606232621_qzxc GGGACAGCTAAGGAGATGGCCGTGGACTGTTG(+ATATAT)GATCCCTGAGGTTGGGCTGGGCTCTTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606232620_8srx TGTCCCTCTGACTGGATACAACCTAGTAAATG(+ATATAT)GTACCTTCCAGCTGGGCCTGTTTCTGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606232618_1i8l GAAGGAGCAGCAGAAACAGGCCCAGCTGGAAG(+ATATAT)GTACCATTTACTAGGTTGTATCCAGTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606232616_quyn TTGTCCCCAGGATACAGCAGATGCTCAACAGG(+ATATAT)GTACCAGAGACACTGTCTGTCTGGAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606232614_5ezd CCGCCAGAGCCTCCAGACAGACAGTGTCTCTG(+ATATAT)GTACCCTGTTGAGCATCTGCTGTATCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606232612_qn7z TTTTCCTTTCCAGAAACATGTTTGGGGCCGTG(+ATATAT)GATCCGAACAGGCAACAGGGGTGCTCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240606232607_nmzm ATCTTTGGCAGAGCACCCCTGTTGCCTGTTCG(+ATATAT)GATCCACGGCCCCAAACATGTTTCTGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606232605_n3wt CAAGGAAGGGCCCTCTGTCCCCTGATATGCTG(+ATATAT)GTACCATTGTTTGTGTCCAGGGGATGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606232603_ft3c GGTGAGCTGGCATCCCCTGGACACAAACAATG(+ATATAT)GTACCAGCATATCAGGGGACAGAGGGCCCTT NGG 10 8 17 8 24 0 100 5 10