Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232926_h9g5 CCCTAAGGAGACGTCAGGGTGCCGTCGGGCTG(+ATATAT)GATCCTAGGTGGACACTTGGTACCTCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606232923_nzfc CAGCAACAGAGAGGTACCAAGTGTCCACCTAG(+ATATAT)GATCCAGCCCGACGGCACCCTGACGTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606232921_lce6 CCTGGCCTACAATCCCTTGTCAGGCGTCCAGG(+ATATAT)GTACCACACTGAGAACAGCTGGCATTTGGAT NGG 10 8 17 8 24 0 100 5 10
    240606232919_3juf GAACCATCCAAATGCCAGCTGTTCTCAGTGTG(+ATATAT)GTACCCTGGACGCCTGACAAGGGATTGTAGG NGG 10 8 17 8 24 0 100 5 10
    240606232918_fy2z TCCATGCTCTCCCAATAAACATGTTGCAGAAG(+ATATAT)GATCCTGTTGTGGCATCTTACTTGCTGGCGA NGG 10 8 17 8 24 0 100 5 10
    240606232916_ocoe CCCACTCGCCAGCAAGTAAGATGCCACAACAG(+ATATAT)GATCCTTCTGCAACATGTTTATTGGGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606232914_esws TCCATGCTCTCCCAATAAACATGTTGCAGAAG(+ATATAT)GATCCTGTTGTGGCATCTTACTTGCTGGCGA NGG 10 8 17 8 24 0 100 5 10
    240606232913_pkxh CCCACTCGCCAGCAAGTAAGATGCCACAACAG(+ATATAT)GATCCTTCTGCAACATGTTTATTGGGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606232911_dxer AAACTCTAGCAAGTGGGAGCCTATCTTTGAAG(+ATATAT)GTACCCTGCTGCTCACACTGCTTAGCCCACG NGG 10 8 17 8 24 0 100 5 10
    240606232909_20m1 GTGGGCGTGGGCTAAGCAGTGTGAGCAGCAGG(+ATATAT)GTACCTTCAAAGATAGGCTCCCACTTGCTAG NGG 10 8 17 8 24 0 100 5 10