Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606233619_ouyq TGGTTACCTTGCCCTGAAAGATGCAGCCAGGG(+ATATAT)GTACCTGGGTTAACCCAAGGGCCCAAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240606233616_k99k AGACCAGGGCTTGGGCCCTTGGGTTAACCCAG(+ATATAT)GTACCCCTGGCTGCATCTTTCAGGGCAAGGT NGG 10 8 17 8 24 0 100 5 10
    240606233614_ut9m TGGGTGAGGAGGTACACACAGGGCTCAGGATG(+ATATAT)GATCCCCTATGGGGAGGAGGGCAGACAATTT NGG 10 8 17 8 24 0 100 5 10
    240606233612_isw3 AGTGCAAATTGTCTGCCCTCCTCCCCATAGGG(+ATATAT)GATCCATCCTGAGCCCTGTGTGTACCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606233611_gmh3 TGGGAATCACACTTTAAAAGGGTCAGAATATG(+ATATAT)GATCCCGAGGGAAAGAATGCTCCGCACTGTT NGG 10 8 17 8 24 0 100 5 10
    240606233608_vc0o TCATGAACAGTGCGGAGCATTCTTTCCCTCGG(+ATATAT)GATCCATATTCTGACCCTTTTAAAGTGTGAT NGG 10 8 17 8 24 0 100 5 10
    240606233606_jh8y TGCAATGCCTCAGAGACAAGAAGAGTGCATTG(+ATATAT)GATCCTAGGGGGCTGCAGTTACAGTCTGTTG NGG 10 8 17 8 24 0 100 5 10
    240606233603_g6zg ACTCACAACAGACTGTAACTGCAGCCCCCTAG(+ATATAT)GATCCAATGCACTCTTCTTGTCTCTGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606233601_ooee TGCAATATTTGTACACATATACAAGTCCTGAG(+ATATAT)GATCCTAATTTCAGCTCAGAGATCGAATTGG NGG 10 8 17 8 24 0 100 5 10
    240606233600_izny CTGACCCAATTCGATCTCTGAGCTGAAATTAG(+ATATAT)GATCCTCAGGACTTGTATATGTGTACAAATA NGG 10 8 17 8 24 0 100 5 10