Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606230155_cyem TCATCTGGTTGAAAACAGAAATCCAGAACCTG(+ATATAT)GATCCCTAGGTCTTAGGGCTGACCCACACCA NGG 10 8 17 8 24 0 100 5 10
    240606230153_wsd7 GAACATGGTGTGGGTCAGCCCTAAGACCTAGG(+ATATAT)GATCCAGGTTCTGGATTTCTGTTTTCAACCA NGG 10 8 17 8 24 0 100 5 10
    240606230151_nhsg CTAGACTGCAGAGCTTCCAATAAAGCCGCTTG(+ATATAT)GTACCGTGGATCTGTCTGTCAGCCTCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240606230148_hz45 AGAGAGAACAGAGGCTGACAGACAGATCCACG(+ATATAT)GTACCAAGCGGCTTTATTGGAAGCTCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606230146_gv9c TGGAGGGCTTGGACATATCCTTTTTCTTGCAG(+ATATAT)GTACCTTGAGAACTCAAGGATGTAACATCCA NGG 10 8 17 8 24 0 100 5 10
    240606230144_5265 GTCCTTGGATGTTACATCCTTGAGTTCTCAAG(+ATATAT)GTACCTGCAAGAAAAAGGATATGTCCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606230143_ydv1 GTTGAGGAGAGCAGCATATTCATGGAGCGCTG(+ATATAT)GATCCTCTGGGGCAGCCCCCAGCGGAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606230140_fsqn CTGATTCACCTCCGCTGGGGGCTGCCCCAGAG(+ATATAT)GATCCAGCGCTCCATGAATATGCTGCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240606230138_6upg CTAGACTGCAGTGCTTCCAATAAAGCTGCTTG(+ATATAT)GTACCGTGGATCTGTCTGTCGGTTGTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240606230136_lqug GAGAGAACAGACAACCGACAGACAGATCCACG(+ATATAT)GTACCAAGCAGCTTTATTGGAAGCACTGCAG NGG 10 8 17 8 24 0 100 5 10