Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606230133_atcj GTCATGTAACCCCTATGTCAGTGTCACCTTTG(+ATATAT)GTACCCCACCTCTTTGAGTCAATTCTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240606230131_b374 GAAAAGGAGCAGAATTGACTCAAAGAGGTGGG(+ATATAT)GTACCAAAGGTGACACTGACATAGGGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240606230129_kvm1 TTGATTCTTTCAGGATGTGACGCCCACTCTGG(+ATATAT)GATCCAGTCCTGGTTGATGTCAGCATGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606230126_8gfc TGGAGGTGGCATGCTGACATCAACCAGGACTG(+ATATAT)GATCCCAGAGTGGGCGTCACATCCTGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606230125_0k00 CCTGAGTATACCTGAGTCAGAGCGTGGAATCG(+ATATAT)GATCCAAGAAGTCAGTCGGCCTGGGGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606230123_h3xo ATCGACTGGACCCCAGGCCGACTGACTTCTTG(+ATATAT)GATCCGATTCCACGCTCTGACTCAGGTATAC NGG 10 8 17 8 24 0 100 5 10
    240606230121_ffrl CTATGACATCTGTGTGTCTGTGGTCTCACGAG(+ATATAT)GTACCTGGAGGTGTATGTGCACGGACATGGG NGG 10 8 17 8 24 0 100 5 10
    240606230119_zu14 TACTTCCCATGTCCGTGCACATACACCTCCAG(+ATATAT)GTACCTCGTGAGACCACAGACACACAGATGT NGG 10 8 17 8 24 0 100 5 10
    240606230117_vm1i GAAAGGAAGGAAAGGGGTTTTGAGAAGAAAAG(+ATATAT)GATCCAAGCAATGGGGGCAGTTCTCACACCT NGG 10 8 17 8 24 0 100 5 10
    240606230115_3ijo CTCTGAGGTGTGAGAACTGCCCCCATTGCTTG(+ATATAT)GATCCTTTTCTTCTCAAAACCCCTTTCCTTC NGG 10 8 17 8 24 0 100 5 10