Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606230113_vs2j CTGACAGTGCCTTTCTGTCTTCAAAGACACTG(+ATATAT)GTACCAGCCAGGCAGTCAGGTGAACTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606230111_3oll GCTGGGAAGGAGTTCACCTGACTGCCTGGCTG(+ATATAT)GTACCAGTGTCTTTGAAGACAGAAAGGCACT NGG 10 8 17 8 24 0 100 5 10
    240606230108_nm7c TATTGCTGTGTGTGCAGGACAGATGTTACCAG(+ATATAT)GTACCCTCAAATCATTCTCTACCTTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240606230106_5wb3 GCTTCAAAAATAAGGTAGAGAATGATTTGAGG(+ATATAT)GTACCTGGTAACATCTGTCCTGCACACACAG NGG 10 8 17 8 24 0 100 5 10
    240606230104_ed48 AGCACCTTCCTGGAGCCCCGTGCCCTTGCTGG(+ATATAT)GATCCTGAGACAACTGTCAGCTGAGCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606230102_6qyg TTCCCACAGGGCTCAGCTGACAGTTGTCTCAG(+ATATAT)GATCCCAGCAAGGGCACGGGGCTCCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606230059_oo24 ACAGACCAGGCAGGGCTGGCTGGAGTTGAGGG(+ATATAT)GATCCCGGTCCAAGAGAGAGAGAGCGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606230057_kk0c CAGCGCCCAGCGCTCTCTCTCTCTTGGACCGG(+ATATAT)GATCCCCTCAACTCCAGCCAGCCCTGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606230055_6ufo CGAGAGGGCGTCAAAGAGGAAGCCTGGGCTGG(+ATATAT)GATCCTTAGGCTCAGCTGTCTGTCTAATCCC NGG 10 8 17 8 24 0 100 5 10
    240606230053_1zxz GCTCTGGGATTAGACAGACAGCTGAGCCTAAG(+ATATAT)GATCCCAGCCCAGGCTTCCTCTTTGACGCCC NGG 10 8 17 8 24 0 100 5 10