Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606222725_mzj4 GCTCAATGTTTTTTGGTTGCATCATGCAAGTG(+ATATAT)GTACCAGCTAGAAACAAGGTGTGAAATAAAA NGG 10 8 17 8 24 0 100 5 10
    240606222723_9fyc AACAGTTTTATTTCACACCTTGTTTCTAGCTG(+ATATAT)GTACCACTTGCATGATGCAACCAAAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240606222721_9l7z TCTTTTGGAGTTGTCCAAAAGATTGTGAGTTG(+ATATAT)GATCCCAGACATTGGACAGTTGGAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240606222719_s6ei TAAAAATTAAACTCCAACTGTCCAATGTCTGG(+ATATAT)GATCCAACTCACAATCTTTTGGACAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240606222717_mv9q ACAGAGGAAGTGTTCTAGAGATAATCAAGGTG(+ATATAT)GTACCTTGTGCTGTGGCTGGACTTGGTAGTG NGG 10 8 17 8 24 0 100 5 10
    240606222716_a2oe TTACACACTACCAAGTCCAGCCACAGCACAAG(+ATATAT)GTACCACCTTGATTATCTCTAGAACACTTCC NGG 10 8 17 8 24 0 100 5 10
    240606222714_1pp6 TTAATCCTCTAGGAGTTAATCCTCTAGGACTG(+ATATAT)GTACCTACTCAGTTATGGCAAATAGACGTTA NGG 10 8 17 8 24 0 100 5 10
    240606222713_o4cy ATAAGTAACGTCTATTTGCCATAACTGAGTAG(+ATATAT)GTACCAGTCCTAGAGGATTAACTCCTAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606222711_sq7o GCTCAATGTTTTTTGGTTGCATCATGCAAGTG(+ATATAT)GTACCAGCTAGAAACAAGGTGTGAAATAAAA NGG 10 8 17 8 24 0 100 5 10
    240606222709_digs AACAGTTTTATTTCACACCTTGTTTCTAGCTG(+ATATAT)GTACCACTTGCATGATGCAACCAAAAAACAT NGG 10 8 17 8 24 0 100 5 10