Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606222651_jxpc TCTTTTGGAGTTGTCCAAAAGATCGTGAGTTG(+ATATAT)GATCCCAGACATTGGACAGTTGGAGTTTCAT NGG 10 8 17 8 24 0 100 5 10
    240606222650_jqxy TAAAAATGAAACTCCAACTGTCCAATGTCTGG(+ATATAT)GATCCAACTCACGATCTTTTGGACAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240606222648_riqw GTGCTGTGGCTGGACTTGGTAACGTGTAAGAG(+ATATAT)GTACCCAGATGGTACTGGTTTTGAAAGCATA NGG 10 8 17 8 24 0 100 5 10
    240606222646_4vlc CCTTTTATGCTTTCAAAACCAGTACCATCTGG(+ATATAT)GTACCTCTTACACGTTACCAAGTCCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606222645_s4to GCCCTTGGAGGAGCCCAAAGGATCGTGAGTTG(+ATATAT)GATCCCATACTTTGGATAGTTGGAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240606222643_nxee CCAAAATTAAACTCCAACTATCCAAAGTATGG(+ATATAT)GATCCAACTCACGATCCTTTGGGCTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240606222640_x3la TTTTTGGGCTTATTTTGAAGCCACTCCCCAAG(+ATATAT)GATCCACATTTATTCATGAAAGCAGATATCC NGG 10 8 17 8 24 0 100 5 10
    240606222639_6yga GGTCTGGATATCTGCTTTCATGAATAAATGTG(+ATATAT)GATCCTTGGGGAGTGGCTTCAAAATAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240606222636_qmhf AAATTCAATTTCATAGACTACTGCCCAGCTAG(+ATATAT)GATCCAGGTCTCAAGCACACAGATGCCTCGC NGG 10 8 17 8 24 0 100 5 10
    240606222634_oo0j AGTTAGCGAGGCATCTGTGTGCTTGAGACCTG(+ATATAT)GATCCTAGCTGGGCAGTAGTCTATGAAATTG NGG 10 8 17 8 24 0 100 5 10