Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606222535_alxu GCCAGGATCTCTGCTTTTTCCCTTTGCATCCG(+ATATAT)GTACCCCTAACAAAGATTTGTTTCTGCACTA NGG 10 8 17 8 24 0 100 5 10
    240606222533_koxh TGAAATAGTGCAGAAACAAATCTTTGTTAGGG(+ATATAT)GTACCGGATGCAAAGGGAAAAAGCAGAGATC NGG 10 8 17 8 24 0 100 5 10
    240606222530_zf9l TGTTTCCTCTGACTTGTTGCACCTGATTTTAG(+ATATAT)GATCCAAAACTTGTTTTGTTGTTACTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606222528_r99b AAAAGAAAGAAGTAACAACAAAACAAGTTTTG(+ATATAT)GATCCTAAAATCAGGTGCAACAAGTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606222526_zw0h GCCAGCAGGAACTTTACAGTCCTGCCCAGCGG(+ATATAT)GATCCTTCCACTGGAAATCAATAGAAATCAT NGG 10 8 17 8 24 0 100 5 10
    240606222524_48u2 GATTCATGATTTCTATTGATTTCCAGTGGAAG(+ATATAT)GATCCCGCTGGGCAGGACTGTAAAGTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606222523_a1xy CCACAGTTAGCTCAAGTGACAGGACTCAGACG(+ATATAT)GATCCTACTGCTTTGGTCATCTGGGGAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606222521_stoo TTTCCAGCTTCCCCAGATGACCAAAGCAGTAG(+ATATAT)GATCCGTCTGAGTCCTGTCACTTGAGCTAAC NGG 10 8 17 8 24 0 100 5 10
    240606222518_6g15 CAGGGTGGCATGCTTACTGGCTAAAGTGCCAG(+ATATAT)GTACCCAAGGGCACCCATGTCCCTCCCTACT NGG 10 8 17 8 24 0 100 5 10
    240606222516_z3g9 TTAAGAGTAGGGAGGGACATGGGTGCCCTTGG(+ATATAT)GTACCTGGCACTTTAGCCAGTAAGCATGCCA NGG 10 8 17 8 24 0 100 5 10