Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606222458_68pi GCTGTCTTCAGACCCATTAGAAGAGGGCATCG(+ATATAT)GATCCCATTATAGATGGTTATAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606222456_t3z7 ACCACATGGTGGCTTATAACCATCTATAATGG(+ATATAT)GATCCGATGCCCTCTTCTAATGGGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606222454_t67u GGTCAGGAGGACCCAGCGCCTACTGCACCCAG(+ATATAT)GATCCATTAGCACTGTAACAACGAACACCTG NGG 10 8 17 8 24 0 100 5 10
    240606222452_cvqt CCACTCAGGTGTTCGTTGTTACAGTGCTAATG(+ATATAT)GATCCTGGGTGCAGTAGGCGCTGGGTCCTCC NGG 10 8 17 8 24 0 100 5 10
    240606222451_apbk GCAGAACTGAATAAACACAGGGTGTGAGGATG(+ATATAT)GATCCTGCCTGGCTGTGCATCCCTGACTCCA NGG 10 8 17 8 24 0 100 5 10
    240606222449_oy8j GCAGCTGGAGTCAGGGATGCACAGCCAGGCAG(+ATATAT)GATCCATCCTCACACCCTGTGTTTATTCAGT NGG 10 8 17 8 24 0 100 5 10
    240606222448_ezph GTTATCCATCCCAGAACTGATGTTTTTCTAAG(+ATATAT)GTACCGAAACCCCAAGTTGATGTGTGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606222446_vszl AAACACAGGACACACATCAACTTGGGGTTTCG(+ATATAT)GTACCTTAGAAAAACATCAGTTCTGGGATGG NGG 10 8 17 8 24 0 100 5 10
    240606222443_mt2d AAACTTCATTTGTCTCCCCATATAAAAAGGGG(+ATATAT)GTACCAACCAGTTGGCCTGGAGCTGGGGTGC NGG 10 8 17 8 24 0 100 5 10
    240606222442_rso3 CCTCAGCACCCCAGCTCCAGGCCAACTGGTTG(+ATATAT)GTACCCCCTTTTTATATGGGGAGACAAATGA NGG 10 8 17 8 24 0 100 5 10