Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606222347_0zrs GGATGTCCTTCCAGGTCAATAATCTTAGCAGG(+ATATAT)GTACCTTCCTCAGAATGGCCAGAGCTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606222345_j6l5 CTCCTTCTCCAGCTCTGGCCATTCTGAGGAAG(+ATATAT)GTACCCTGCTAAGATTATTGACCTGGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606222343_3d1q CACCAGAAAGAGATCATGATGTGCCAGCCAGG(+ATATAT)GATCCTGCGACCCAGATCTTCTCTCCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240606222342_2pqe AACTGTCTCTGGAGAGAAGATCTGGGTCGCAG(+ATATAT)GATCCCTGGCTGGCACATCATGATCTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240606222340_dpq4 GGGGGGGGGGGCTGGGTTTTTGGAATCTGTAG(+ATATAT)GATCCATAGCACAATCATCTACCACACACCT NGG 10 8 17 8 24 0 100 5 10
    240606222339_dh3g GAAGTAGGTGTGTGGTAGATGATTGTGCTATG(+ATATAT)GATCCTACAGATTCCAAAAACCCAGCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240606222338_9c6d CTCCTCTGGGGAGCCTTTCCATAGCAGCTCAG(+ATATAT)GTACCAAAGAGGCAAGGAAAGAGCAGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240606222336_vdpo CCAGGAACCCCTGCTCTTTCCTTGCCTCTTTG(+ATATAT)GTACCTGAGCTGCTATGGAAAGGCTCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240606222334_4bzt GGGAGCCAGCAGAGACCAAAGGGAAGGAGGAG(+ATATAT)GTACCTGAGGCTTGGCAGGATCCCCAGGGGT NGG 10 8 17 8 24 0 100 5 10
    240606222332_4dp2 CAAGGACCCCTGGGGATCCTGCCAAGCCTCAG(+ATATAT)GTACCTCCTCCTTCCCTTTGGTCTCTGCTGG NGG 10 8 17 8 24 0 100 5 10