Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606222439_ufqm ACCACACAGTGGCTCACAACTATCTGTAATGG(+ATATAT)GATCCGATGCTCTCTTCCGGCTTGCAGGGTT NGG 10 8 17 8 24 0 100 5 10
    240606222438_ikpj CATGTAACCCTGCAAGCCGGAAGAGAGCATCG(+ATATAT)GATCCCATTACAGATAGTTGTGAGCCACTGT NGG 10 8 17 8 24 0 100 5 10
    240606222437_opxw GTGGGGACAGTGGGTGACTCAGAACTCTGCTG(+ATATAT)GTACCTGGGGGTGGAGCTATCACTTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240606222433_jknm CCCTCTGGAGGAAGTGATAGCTCCACCCCCAG(+ATATAT)GTACCAGCAGAGTTCTGAGTCACCCACTGTC NGG 10 8 17 8 24 0 100 5 10
    240606222432_lotu TGAAAGTATTAGAGGCTGGAGGGACATTATTG(+ATATAT)GTACCCTAAGACGTACCCCATGTTTAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240606222430_3k0e TTTAATCTGCTAAACATGGGGTACGTCTTAGG(+ATATAT)GTACCAATAATGTCCCTCCAGCCTCTAATAC NGG 10 8 17 8 24 0 100 5 10
    240606222428_cqik CTGATTGACTAAAGTAGCTGGAAAAGGTTGGG(+ATATAT)GATCCGTGTTTATTAGAAACAGTCCAACAAG NGG 10 8 17 8 24 0 100 5 10
    240606222427_hmye CCTTTCTTGTTGGACTGTTTCTAATAAACACG(+ATATAT)GATCCCCAACCTTTTCCAGCTACTTTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606222425_ng0n GGTGAATCGGAAAGGGTGAGAGCAGGGAGGGG(+ATATAT)GATCCCTGGCAGAGCCGTAATATTCCTATGT NGG 10 8 17 8 24 0 100 5 10
    240606222423_m0cb CAGGGACATAGGAATATTACGGCTCTGCCAGG(+ATATAT)GATCCCCCTCCCTGCTCTCACCCTTTCCGAT NGG 10 8 17 8 24 0 100 5 10