Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606222310_01b8 TGTCTTAGTGCAGCAGAAGATAGTTCCAGCAG(+ATATAT)GTACCGGCTGGGCTTGGTGGCTTTCCAAGCG NGG 10 8 17 8 24 0 100 5 10
    240606222308_e1uz ACGTGCGCTTGGAAAGCCACCAAGCCCAGCCG(+ATATAT)GTACCTGCTGGAACTATCTTCTGCTGCACTA NGG 10 8 17 8 24 0 100 5 10
    240606222307_o0is CCATGAATATTAGTGTCCAACAGGTGGAGTCG(+ATATAT)GTACCCACATTCCTACCCCGCCCCAGACCTG NGG 10 8 17 8 24 0 100 5 10
    240606222305_3c57 AGTGCCAGGTCTGGGGCGGGGTAGGAATGTGG(+ATATAT)GTACCGACTCCACCTGTTGGACACTAATATT NGG 10 8 17 8 24 0 100 5 10
    240606222303_karv GGGTGCAGGCAGCTGTAGAATCCCAAAGAAGG(+ATATAT)GATCCAAACAATGTTTGAAGACCCCACCCCA NGG 10 8 17 8 24 0 100 5 10
    240606222302_n1in TAAATTGGGGTGGGGTCTTCAAACATTGTTTG(+ATATAT)GATCCCTTCTTTGGGATTCTACAGCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606222300_r7oh CAAGTAAATGGCCTTGAAGTGGCCATGCCTTG(+ATATAT)GTACCGAGCAGTCAAACTGATGAACACGGCA NGG 10 8 17 8 24 0 100 5 10
    240606222259_dzo8 ACAAATGCCGTGTTCATCAGTTTGACTGCTCG(+ATATAT)GTACCAAGGCATGGCCACTTCAAGGCCATTT NGG 10 8 17 8 24 0 100 5 10
    240606222257_xe8z CTATCTAGATGGACCTGTATAATGTCTGCCTG(+ATATAT)GTACCTGAGATCTAGCCTCTGCACTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606222256_pnlw ATTTGCCACTAAGTGCAGAGGCTAGATCTCAG(+ATATAT)GTACCAGGCAGACATTATACAGGTCCATCTA NGG 10 8 17 8 24 0 100 5 10