Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606222157_8mwm CCTCCAGAGCTCACCTTCCACGCAGTGATGAG(+ATATAT)GATCCTGTCAGGGACCCTACCTCACAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240606222156_07hx AGCTGACTGCTGTGAGGTAGGGTCCCTGACAG(+ATATAT)GATCCTCATCACTGCGTGGAAGGTGAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606222154_xj89 GCAGGGTGGCAGTCACCTCCCCTGTCCACGTG(+ATATAT)GATCCATCCCTGCCATTGTGTGAGTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606222153_xdeh CTATCCCTCTGACTCACACAATGGCAGGGATG(+ATATAT)GATCCACGTGGACAGGGGAGGTGACTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606222151_cb2u ACGGAGAACCAAGGAAGACAATGTTTCATCCG(+ATATAT)GATCCAGAGGCGCATCAAATTCAACAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240606222149_p9ag GTGGAGCTGCTGTTGAATTTGATGCGCCTCTG(+ATATAT)GATCCGGATGAAACATTGTCTTCCTTGGTTC NGG 10 8 17 8 24 0 100 5 10
    240606222146_xf6a CTCTGGAGTGAAAACATAAAACCTTGTCCCTG(+ATATAT)GTACCGTGAAGCCATGCAAGAGACGAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606222144_hhme TTTTCTCACCTCGTCTCTTGCATGGCTTCACG(+ATATAT)GTACCAGGGACAAGGTTTTATGTTTTCACTC NGG 10 8 17 8 24 0 100 5 10
    240606222143_002f ACGTATCCACAGTGCAATGTTGGCTCAGCCAG(+ATATAT)GATCCACCTTGCATACATACTAAAGAATGAC NGG 10 8 17 8 24 0 100 5 10
    240606222140_xfwt GATCAGTCATTCTTTAGTATGTATGCAAGGTG(+ATATAT)GATCCTGGCTGAGCCAACATTGCACTGTGGA NGG 10 8 17 8 24 0 100 5 10