Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606222056_km1r TGCATTTTCCTGACTGATGATTGATGTGGGAG(+ATATAT)GATCCATCCCAATGTGGGCAACTATAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606222055_cttb CCTGCTTTCTTATAGTTGCCCACATTGGGATG(+ATATAT)GATCCTCCCACATCAATCATCAGTCAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240606222053_g7ki CAAGCAAACCATTCCACCTCAGAGCATATTTG(+ATATAT)GATCCAATCTCTTTATTGTCAGGTTCCTCCC NGG 10 8 17 8 24 0 100 5 10
    240606222052_cacs AGGGTGGGAGGAACCTGACAATAAAGAGATTG(+ATATAT)GATCCAAATATGCTCTGAGGTGGAATGGTTT NGG 10 8 17 8 24 0 100 5 10
    240606222051_yd46 GCATGTACATCTGTGTACCATGCGTGTGTGTG(+ATATAT)GTACCAGCAGAAGTCAGCAGAGGGCGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240606222049_b8ch AGGAACTCAGCGCCCTCTGCTGACTTCTGCTG(+ATATAT)GTACCACACACACGCATGGTACACAGATGTA NGG 10 8 17 8 24 0 100 5 10
    240606222047_rs9r CACAGCACGTGTCAAGCTTGTATGAGACGCAG(+ATATAT)GATCCTATCACCAGCACTGAGAATAAAACTT NGG 10 8 17 8 24 0 100 5 10
    240606222045_4vzy ACTTGAAGTTTTATTCTCAGTGCTGGTGATAG(+ATATAT)GATCCTGCGTCTCATACAAGCTTGACACGTG NGG 10 8 17 8 24 0 100 5 10
    240606222043_utrq GACACATCCTGGTGTGTCCACATGTGTGCATG(+ATATAT)GATCCATGTGTGCGCATTGGGGTGGGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240606222041_njjk AGAGCCCACCCCCACCCCAATGCGCACACATG(+ATATAT)GATCCATGCACACATGTGGACACACCAGGAT NGG 10 8 17 8 24 0 100 5 10