Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606222021_1j28 AAGAATGTGTGTCATGGTAGAGGGGTTCCCAG(+ATATAT)GATCCTCTCCTGCCAGATGCTTGGCTGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606222019_95lr GACCTGCAGCAGCCAAGCATCTGGCAGGAGAG(+ATATAT)GATCCTGGGAACCCCTCTACCATGACACACA NGG 10 8 17 8 24 0 100 5 10
    240606222016_lqq5 CACCTACCCCGATGCCCGAGTTCAACTCCCAG(+ATATAT)GATCCACTTGGTGGAAGGAGACCACTGATCC NGG 10 8 17 8 24 0 100 5 10
    240606222013_et10 TGTGGGGATCAGTGGTCTCCTTCCACCAAGTG(+ATATAT)GATCCTGGGAGTTGAACTCGGGCATCGGGGT NGG 10 8 17 8 24 0 100 5 10
    240606222011_icz6 CCAGTTGGTACACTGTTTTCTGAAAGAGGTGG(+ATATAT)GATCCAAAAGAGCTGTTTCTAGCTGCACTCC NGG 10 8 17 8 24 0 100 5 10
    240606222010_ysb7 TGCTTGGAGTGCAGCTAGAAACAGCTCTTTTG(+ATATAT)GATCCCACCTCTTTCAGAAAACAGTGTACCA NGG 10 8 17 8 24 0 100 5 10
    240606222008_vqwg TGCCAGTCTGTGTCTGTCTAATCAGAAGGATG(+ATATAT)GATCCCTGTACCTAACATTCCATATCACTAC NGG 10 8 17 8 24 0 100 5 10
    240606222006_2l6y TCAGTGTAGTGATATGGAATGTTAGGTACAGG(+ATATAT)GATCCATCCTTCTGATTAGACAGACACAGAC NGG 10 8 17 8 24 0 100 5 10
    240606222005_l9bh AAGGGCTATTCAAGCTCCCAAGAAAATCTGTG(+ATATAT)GATCCATGGGTTACAGATTAAAGTTCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240606221959_i2w2 ACCCAGTTTGGAACTTTAATCTGTAACCCATG(+ATATAT)GATCCACAGATTTTCTTGGGAGCTTGAATAG NGG 10 8 17 8 24 0 100 5 10