Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606222139_e4mw ATGTAGTGCCTTTTTTGTGTTCTGAGGGAGTG(+ATATAT)GTACCCTGAGGTCTCGTCGTGTAGTTTTAGA NGG 10 8 17 8 24 0 100 5 10
    240606222137_wi25 TTAACTCTAAAACTACACGACGAGACCTCAGG(+ATATAT)GTACCACTCCCTCAGAACACAAAAAAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240606222134_2z98 TGCCAAGAGCTGGTAAGCGACACAACCCATGG(+ATATAT)GTACCAGGGATTCTGGCTGACACAAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240606222131_6n22 TCAGGTTCACCTTGTGTCAGCCAGAATCCCTG(+ATATAT)GTACCCATGGGTTGTGTCGCTTACCAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606222130_887r TACTCACGGTCTCTGTTTTCTCACTCTCTGAG(+ATATAT)GTACCACAGGCAGGCAGCCCTTGAGCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240606222127_5c6d CATTGGCTGAGCTCAAGGGCTGCCTGCCTGTG(+ATATAT)GTACCTCAGAGAGTGAGAAAACAGAGACCGT NGG 10 8 17 8 24 0 100 5 10
    240606222125_afny AATAGTGAGCGGGGAAAGGAGAATAGGAAGTG(+ATATAT)GATCCTTCCAGAACCTACCTCTCTCTGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606222124_is20 GTTGAGCTTCAGAGAGAGGTAGGTTCTGGAAG(+ATATAT)GATCCACTTCCTATTCTCCTTTCCCCGCTCA NGG 10 8 17 8 24 0 100 5 10
    240606222123_rbrb CCTCTCTTTACGTGAGATACTGTGGCGTGCTG(+ATATAT)GTACCATCCAAGCCTCGGCCCGGTGTCTATC NGG 10 8 17 8 24 0 100 5 10
    240606222121_ahbv GAGGGGATAGACACCGGGCCGAGGCTTGGATG(+ATATAT)GTACCAGCACGCCACAGTATCTCACGTAAAG NGG 10 8 17 8 24 0 100 5 10