Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606221940_pk2u TGGGGGTTCAGGCTCCAGGATCAATTTAGAGG(+ATATAT)GATCCTTGAACCCAGCCCCGTGAACAATCTA NGG 10 8 17 8 24 0 100 5 10
    240606221939_it3m TATGATAGATTGTTCACGGGGCTGGGTTCAAG(+ATATAT)GATCCCTCTAAATTGATCCTGGAGCCTGAAC NGG 10 8 17 8 24 0 100 5 10
    240606221938_gbdf GACTAACATGCCCAGGTGGTGTCGGCTCAGAG(+ATATAT)GATCCTAGCACCATCTTAAGCAGGTGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606221936_huwz CCCTGCCTCCCACCTGCTTAAGATGGTGCTAG(+ATATAT)GATCCTCTGAGCCGACACCACCTGGGCATGT NGG 10 8 17 8 24 0 100 5 10
    240606221934_cqds TGTTCAGTAAACTCTGGCTGCTTTGAGGAGGG(+ATATAT)GATCCTGCTGTCCCAGGGAACCAGGACATGT NGG 10 8 17 8 24 0 100 5 10
    240606221932_lqq8 CAAGGACATGTCCTGGTTCCCTGGGACAGCAG(+ATATAT)GATCCCCTCCTCAAAGCAGCCAGAGTTTACT NGG 10 8 17 8 24 0 100 5 10
    240606221931_eil0 GCAGTCCCCACGCAGGGTGGCTTCCTGTCGAG(+ATATAT)GATCCAGTGGGCCCAGCGTTGGTCCACCCAC NGG 10 8 17 8 24 0 100 5 10
    240606221928_154o ACTGAGTGGGTGGACCAACGCTGGGCCCACTG(+ATATAT)GATCCTCGACAGGAAGCCACCCTGCGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606221926_x2jn GGCAGGTATCAGAGGTGAAGGTATTCTGGTGG(+ATATAT)GATCCCCCTCCCAAGGGACCTTGGCTGGAGT NGG 10 8 17 8 24 0 100 5 10
    240606221924_sacw CTAGGACTCCAGCCAAGGTCCCTTGGGAGGGG(+ATATAT)GATCCCACCAGAATACCTTCACCTCTGATAC NGG 10 8 17 8 24 0 100 5 10