Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606222243_6m1l ACATACGCAGAGCCCCGGAGGATGCTGCCCGG(+ATATAT)GTACCTGCATCTCTCCAGGGGTTTGTCCACA NGG 10 8 17 8 24 0 100 5 10
    240606222242_rk2b GTGTTCTCCCTCCATCCACTTAGGCCCAGATG(+ATATAT)GTACCTAGTAAACAAGCCATAAACACATGGG NGG 10 8 17 8 24 0 100 5 10
    240606222240_uu72 CAAGTCCCATGTGTTTATGGCTTGTTTACTAG(+ATATAT)GTACCATCTGGGCCTAAGTGGATGGAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606222238_0cqa TTTTGATTTGGGTCCAACCAAAACCTTCCTTG(+ATATAT)GATCCATCTCTTGCTCACTTACACAGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606222237_boxg CCATGGAGGCCTGTGTAAGTGAGCAAGAGATG(+ATATAT)GATCCAAGGAAGGTTTTGGTTGGACCCAAAT NGG 10 8 17 8 24 0 100 5 10
    240606222235_yjqe GCTGTGAGGAAGGTCCTGTGCCGACACAGTGG(+ATATAT)GTACCTGGCAAGGGTAAGAGGAGAGCCATCA NGG 10 8 17 8 24 0 100 5 10
    240606222234_nf6b GCTGGTGATGGCTCTCCTCTTACCCTTGCCAG(+ATATAT)GTACCCACTGTGTCGGCACAGGACCTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606222232_8jxn GGGTCTCTGGGTGATGAGGTCAGAGTCTCTGG(+ATATAT)GATCCCCTGAAATCCCGGAGAAGAGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606222230_70ze TTCTTCCCAGGCTCTTCTCCGGGATTTCAGGG(+ATATAT)GATCCCAGAGACTCTGACCTCATCACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606222229_pmpu TCACAACCATCTTTGTAACTCCAGTTTCAAGG(+ATATAT)GATCCAACCCCCTCTCCTGACCTCAAACACC NGG 10 8 17 8 24 0 100 5 10