Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606221809_5ehs ACTCCATAGATCTCAGTAATTCTGGAAACAAG(+ATATAT)GTACCATGACATGCAGGTCATCATTGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606221807_x7yh TTTGGTTTTCCAATGATGACCTGCATGTCATG(+ATATAT)GTACCTTGTTTCCAGAATTACTGAGATCTAT NGG 10 8 17 8 24 0 100 5 10
    240606221806_viww AAAAGGATATACCTTCTTCTCAGCACCTCATG(+ATATAT)GTACCTTCTCCAAAATTGACCACATAATAGG NGG 10 8 17 8 24 0 100 5 10
    240606221803_r12s TGTGACCTATTATGTGGTCAATTTTGGAGAAG(+ATATAT)GTACCATGAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240606221801_yj4n ACGAAATGGACAAATTTCTGGACAGATACCAG(+ATATAT)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10
    240606221800_2tcr TAGAAGGTCAACTTGATCCTGATTCAACTTTG(+ATATAT)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606221759_jovi TATCTGAAACATTAACCAGTTCATCTTCATAG(+ATATAT)GATCCATTTGAAGCCAAATCATGTCATGGTG NGG 10 8 17 8 24 0 100 5 10
    240606221757_rz07 GTGTGCACCATGACATGATTTGGCTTCAAATG(+ATATAT)GATCCTATGAAGATGAACTGGTTAATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240606221755_axn3 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606221754_bo62 TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+ATATAT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10