Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606221751_gq99 GGATGGACCATGTAGAGACTGCCTTATCCAGG(+ATATAT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606221750_6pg0 GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+ATATAT)GATCCCTGGATAAGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606221748_pf0g TTTGGTTTTCCGATGATGACCTGCATGTCATG(+ATATAT)GTACCTTGTTTCCAGAATTATTGAGATCTAT NGG 10 8 17 8 24 0 100 5 10
    240606221747_evjl ACTCCATAGATCTCAATAATTCTGGAAACAAG(+ATATAT)GTACCATGACATGCAGGTCATCATCGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606221745_gh8t ACTCCATAGATCTCAATAATTCTGGAAACAAG(+ATATAT)GTACCATGACATGCAGGTCATCATCGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606221744_39ev TTTGGTTTTCCGATGATGACCTGCATGTCATG(+ATATAT)GTACCTTGTTTCCAGAATTATTGAGATCTAT NGG 10 8 17 8 24 0 100 5 10
    240606221743_c2i8 AACAAAAAACAAAAAACACTGGGATAAAGGAG(+ATATAT)GTACCCAAGAATCAGTGGAGTTTACCCTAGC NGG 10 8 17 8 24 0 100 5 10
    240606221741_5fck TCACAGCTAGGGTAAACTCCACTGATTCTTGG(+ATATAT)GTACCTCCTTTATCCCAGTGTTTTTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606221740_k37e CAGGAATAGAAATCCTGACCAAGACATTGTTG(+ATATAT)GTACCAGCATAGTGGGGCATTCCTGTGACAA NGG 10 8 17 8 24 0 100 5 10
    240606221738_h264 TCAGGTTGTCACAGGAATGCCCCACTATGCTG(+ATATAT)GTACCAACAATGTCTTGGTCAGGATTTCTAT NGG 10 8 17 8 24 0 100 5 10