Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606215808_nhsm AATTCATAGTTATTTATTTTATTCACATTAGG(+ATATAT)GATCCTGAGGAAGTAGAATCTATATAGGACA NGG 10 8 17 8 24 0 100 5 10
    240606215805_ipj2 ATACCTGTCCTATATAGATTCTACTTCCTCAG(+ATATAT)GATCCCTAATGTGAATAAAATAAATAACTAT NGG 10 8 17 8 24 0 100 5 10
    240606215804_l8aq ACCCCAGAGGTAGTATGGCAAAAACGAGCATG(+ATATAT)GATCCTATCAGGTCACAACCGACAAAGTAAT NGG 10 8 17 8 24 0 100 5 10
    240606215802_gpu8 ATTTAATTACTTTGTCGGTTGTGACCTGATAG(+ATATAT)GATCCATGCTCGTTTTTGCCATACTACCTCT NGG 10 8 17 8 24 0 100 5 10
    240606215801_0chc GGCAAAATAAATAGTTAAAACAAGTGATGCAG(+ATATAT)GATCCCATTTCATGCTCATGATCCCATTAAA NGG 10 8 17 8 24 0 100 5 10
    240606215759_m14j AATTCTTTAATGGGATCATGAGCATGAAATGG(+ATATAT)GATCCTGCATCACTTGTTTTAACTATTTATT NGG 10 8 17 8 24 0 100 5 10
    240606215758_m7xu GTTCAGAAGGGATCAAAATTGCCATCTAACAG(+ATATAT)GTACCTTAAAGGATTCCTGGGCATCTACTTC NGG 10 8 17 8 24 0 100 5 10
    240606215757_isz1 TTGTGGAAGTAGATGCCCAGGAATCCTTTAAG(+ATATAT)GTACCTGTTAGATGGCAATTTTGATCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606215755_02lr TTGGGTTGATTTTCCTGAATTGGTTCCTATAG(+ATATAT)GATCCTCCAATTTTGGATCTTCTACAGATCC NGG 10 8 17 8 24 0 100 5 10
    240606215753_m3i9 CTGGGGGATCTGTAGAAGATCCAAAATTGGAG(+ATATAT)GATCCTATAGGAACCAATTCAGGAAAATCAA NGG 10 8 17 8 24 0 100 5 10