Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606215752_pl9z TGAGGCACAGTCTCTGTTCTGGAATTCACAAG(+ATATAT)GTACCTGTAATAAACATCCAAACAAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606215748_p99n ATTTGTGACTCTTGTTTGGATGTTTATTACAG(+ATATAT)GTACCTTGTGAATTCCAGAACAGAGACTGTG NGG 10 8 17 8 24 0 100 5 10
    240606215746_gga1 TCTAATGTAATAATGTTTTTTAAAGCACTCTG(+ATATAT)GTACCTACATGCCATCAATTCCACTTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240606215744_nhbj CTTACGAAGAAAGTGGAATTGATGGCATGTAG(+ATATAT)GTACCAGAGTGCTTTAAAAAACATTATTACA NGG 10 8 17 8 24 0 100 5 10
    240606215742_rvfe ATATAATTTTAGTTTAGCAATAATTTTGACTG(+ATATAT)GTACCAATAACTGTTTTTTAAAATTCCATAC NGG 10 8 17 8 24 0 100 5 10
    240606215741_dctc CATAAGTATGGAATTTTAAAAAACAGTTATTG(+ATATAT)GTACCAGTCAAAATTATTGCTAAACTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606215739_ff7w TAATAACAGACCAGCGATTTTATAGAGGCCAG(+ATATAT)GATCCTGAACTGGGGCATCCTCTCTGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240606215737_op9t GCCTGCACTCCAGAGAGGATGCCCCAGTTCAG(+ATATAT)GATCCTGGCCTCTATAAAATCGCTGGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606215736_m78a TTGGACTTTGTAAGCCTGAAAAAGAATATTTG(+ATATAT)GATCCCTCTACTAAACAAGGGCTATGTATTA NGG 10 8 17 8 24 0 100 5 10
    240606215734_m7sn CTGTATAATACATAGCCCTTGTTTAGTAGAGG(+ATATAT)GATCCAAATATTCTTTTTCAGGCTTACAAAG NGG 10 8 17 8 24 0 100 5 10