Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606215658_5dkw TCTAAGAGTTCTGTGTATATTTCTTCTAGTTG(+ATATAT)GTACCTACACAAATTAGATTTCTCTTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606215657_n4i6 TGGCAAAAGCAAGAGAAATCTAATTTGTGTAG(+ATATAT)GTACCAACTAGAAGAAATATACACAGAACTC NGG 10 8 17 8 24 0 100 5 10
    240606215655_7cr9 GTAAATTAACAACTCAGTTATTAAACTAGCAG(+ATATAT)GATCCTACAATGATACATCTAGTGAAAGTAG NGG 10 8 17 8 24 0 100 5 10
    240606215653_w7ae TAACTCTACTTTCACTAGATGTATCATTGTAG(+ATATAT)GATCCTGCTAGTTTAATAACTGAGTTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240606215652_9wmp ATTAGTTCTGATTCATCCCCGAGGGAAGAGAG(+ATATAT)GTACCCATGGCTTCAGTGAGCACATGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240606215649_ksbt CTGGGCTTGGCATGTGCTCACTGAAGCCATGG(+ATATAT)GTACCTCTCTTCCCTCGGGGATGAATCAGAA NGG 10 8 17 8 24 0 100 5 10
    240606215647_jg0e TGGCAAGTGGCCAGCTGAGCCCAGGTTACTTG(+ATATAT)GTACCCACTCTGCCTTGAGCCTTCCTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240606215644_ldg0 CCCTGGGGGAAGGAAGGCTCAAGGCAGAGTGG(+ATATAT)GTACCAAGTAACCTGGGCTCAGCTGGCCACT NGG 10 8 17 8 24 0 100 5 10
    240606215642_jk95 CCGCTGAGATCCAGCATCCAGCTGTGGCCAGG(+ATATAT)GATCCACCACCTCTGTGTACACCCTCACCCG NGG 10 8 17 8 24 0 100 5 10
    240606215640_ib0e AGCAGCGGGTGAGGGTGTACACAGAGGTGGTG(+ATATAT)GATCCCTGGCCACAGCTGGATGCTGGATCTC NGG 10 8 17 8 24 0 100 5 10