Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606215607_3hhr TCCCTGCAAGGCCAGCCCTGGGACAGTGCCTG(+ATATAT)GTACCCGAGAAACAGTTATACGGGTGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606215605_rreq CAGCGCAGGACACCCGTATAACTGTTTCTCGG(+ATATAT)GTACCAGGCACTGTCCCAGGGCTGGCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240606215602_welr AGATGGAGAGGACCTCAAGAGTCTGACCCTTG(+ATATAT)GTACCATCCGATACTTAACTCCCTCGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240606215600_i7no GAAGAAGGACCGAGGGAGTTAAGTATCGGATG(+ATATAT)GTACCAAGGGTCAGACTCTTGAGGTCCTCTC NGG 10 8 17 8 24 0 100 5 10
    240606215558_ahcr TTCTTCCTCTTCCTCTTCCTCCTTGAATCTGG(+ATATAT)GTACCCTAACCCTGAGAAGAAAACCCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240606215557_2p3k AGAAAACTGAGGGTTTTCTTCTCAGGGTTAGG(+ATATAT)GTACCCAGATTCAAGGAGGAAGAGGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606215555_lq9g GCACAGCACCTACCAGCCCTGGCCCTTCCTTG(+ATATAT)GTACCATCTCCACCACTGCTGGACTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240606215553_h1xd GGGTAGGTGGGAGTCCAGCAGTGGTGGAGATG(+ATATAT)GTACCAAGGAAGGGCCAGGGCTGGTAGGTGC NGG 10 8 17 8 24 0 100 5 10
    240606215552_fzsf ATATGATGAGCCCAAGAGATCTGGGATGAATG(+ATATAT)GATCCCTAGATACGGTAGGCCCAGATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240606215551_w286 GGTGGGGGAGATCTGGGCCTACCGTATCTAGG(+ATATAT)GATCCATTCATCCCAGATCTCTTGGGCTCAT NGG 10 8 17 8 24 0 100 5 10