Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606215445_mui3 AGGGCAAGGCGAACATGAAAGTTAACATGCAG(+ATATAT)GTACCTGGCAGAGTGGGGACCCCAGTGGATG NGG 10 8 17 8 24 0 100 5 10
    240606215443_r2l4 CCCAACATCCACTGGGGTCCCCACTCTGCCAG(+ATATAT)GTACCTGCATGTTAACTTTCATGTTCGCCTT NGG 10 8 17 8 24 0 100 5 10
    240606215441_wphi TGGTGATAAGCTGTGAGGAGTGAATGGACAGG(+ATATAT)GATCCAGAGGGACACAGCAGGCTCCACAAAG NGG 10 8 17 8 24 0 100 5 10
    240606215439_iq4v TTCCTCTTTGTGGAGCCTGCTGTGTCCCTCTG(+ATATAT)GATCCCTGTCCATTCACTCCTCACAGCTTAT NGG 10 8 17 8 24 0 100 5 10
    240606215437_6iae GGAGAAACTGGGTTAAGGCCAGAGAAGTGCCG(+ATATAT)GATCCTGCCCCAGATTTTGACTTTAGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240606215435_x5a9 CATGGGAGCTCTAAAGTCAAAATCTGGGGCAG(+ATATAT)GATCCGGCACTTCTCTGGCCTTAACCCAGTT NGG 10 8 17 8 24 0 100 5 10
    240606215432_adjw GCCCGAGGTTCTGGTGGCCCAGCAACTGCAGG(+ATATAT)GATCCATAAATCCAGAGGCCAGGATTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606215431_ta3j ACTGAGCCTGAATCCTGGCCTCTGGATTTATG(+ATATAT)GATCCCTGCAGTTGCTGGGCCACCAGAACCT NGG 10 8 17 8 24 0 100 5 10
    240606215430_pmu5 ACAAGGGGAAGGAAGCCCAGGTAGAGGGTATG(+ATATAT)GATCCCTCGAACCTGTCAGCAGACGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240606215428_er3i ACCGCGTGTTCCGTCTGCTGACAGGTTCGAGG(+ATATAT)GATCCATACCCTCTACCTGGGCTTCCTTCCC NGG 10 8 17 8 24 0 100 5 10