Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606215328_dx7z GAAACAAAACCAAAACCCAACTTCTCAGTAGG(+ATATAT)GTACCACTAGCTGGCCTGTGGACAACAGTCT NGG 10 8 17 8 24 0 100 5 10
    240606215326_2crt TGAGGAGACTGTTGTCCACAGGCCAGCTAGTG(+ATATAT)GTACCCTACTGAGAAGTTGGGTTTTGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606215324_kv6x CTATCTGGTCTGCCCCTGTGTAGCTCCCATGG(+ATATAT)GATCCCTTAAAATGCATCCTTTTTAATTTTC NGG 10 8 17 8 24 0 100 5 10
    240606215322_uorh GCCCGGAAAATTAAAAAGGATGCATTTTAAGG(+ATATAT)GATCCCATGGGAGCTACACAGGGGCAGACCA NGG 10 8 17 8 24 0 100 5 10
    240606215319_epi4 ATGCGTCCTTGCCCACCTCTCACCAAGCCCCG(+ATATAT)GATCCTTGCGGACTTCCCCAGGTGCCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606215317_g3bl GGGGACAGGGGGCACCTGGGGAAGTCCGCAAG(+ATATAT)GATCCGGGGCTTGGTGAGAGGTGGGCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606215315_7ze1 ACCCGCAGCTCCTAGTCTCTAATGCCTGGTAG(+ATATAT)GATCCCATGGGCCTTTGCGACGCCACGTTAG NGG 10 8 17 8 24 0 100 5 10
    240606215313_kcdj GTTGCCTAACGTGGCGTCGCAAAGGCCCATGG(+ATATAT)GATCCTACCAGGCATTAGAGACTAGGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606215312_3rv3 GGACATCAATCTAAAGCCTCGAGGCCATGTTG(+ATATAT)GATCCACCCTGACCCGGTCCTGTAGCAACTA NGG 10 8 17 8 24 0 100 5 10
    240606215310_8w0q TTTTTTAGTTGCTACAGGACCGGGTCAGGGTG(+ATATAT)GATCCAACATGGCCTCGAGGCTTTAGATTGA NGG 10 8 17 8 24 0 100 5 10