Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606215426_c4z6 ATTCCTGGCCCTAGAAAGGAGGGTCTGCCGAG(+ATATAT)GTACCAGGGGCCTGGCCTCACTGGCTGGACC NGG 10 8 17 8 24 0 100 5 10
    240606215423_zy9n GGTGTGGTCCAGCCAGTGAGGCCAGGCCCCTG(+ATATAT)GTACCTCGGCAGACCCTCCTTTCTAGGGCCA NGG 10 8 17 8 24 0 100 5 10
    240606215421_cil1 GCAGTGGGGGCTTTCTCAGGAGAGATGCCAGG(+ATATAT)GATCCCCAGGGACTGCACACCCCACTATCTC NGG 10 8 17 8 24 0 100 5 10
    240606215418_ecav TCTAGGAGATAGTGGGGTGTGCAGTCCCTGGG(+ATATAT)GATCCCTGGCATCTCTCCTGAGAAAGCCCCC NGG 10 8 17 8 24 0 100 5 10
    240606215417_e1dp TACCTTCTATGAGCGCTGCCTACCTGTTTGTG(+ATATAT)GTACCCTGTGATGGAGGGCCTCACAGCCACA NGG 10 8 17 8 24 0 100 5 10
    240606215415_seg5 ACTAGTGTGGCTGTGAGGCCCTCCATCACAGG(+ATATAT)GTACCACAAACAGGTAGGCAGCGCTCATAGA NGG 10 8 17 8 24 0 100 5 10
    240606215414_rc1j CTCCAATGGCTCTGGCCACTGCTTCTTCATCG(+ATATAT)GATCCTGTTCTCCAGAAGCCCCAGAGAATGG NGG 10 8 17 8 24 0 100 5 10
    240606215413_qeno TCCATCCATTCTCTGGGGCTTCTGGAGAACAG(+ATATAT)GATCCGATGAAGAAGCAGTGGCCAGAGCCAT NGG 10 8 17 8 24 0 100 5 10
    240606215411_hxy6 TGAACACCCAGGCCTTTGGGGATGAGCTGCTG(+ATATAT)GTACCCACTTCTGAAGAGACAGGTGGCCACT NGG 10 8 17 8 24 0 100 5 10
    240606215406_0q18 GCTCCAGTGGCCACCTGTCTCTTCAGAAGTGG(+ATATAT)GTACCAGCAGCTCATCCCCAAAGGCCTGGGT NGG 10 8 17 8 24 0 100 5 10