Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606215526_dxly TGCTGCCGTGTATGTGCTGGGAATTGAACCAG(+ATATAT)GATCCTCTGGATTTGCAGCTAGAACTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240606215525_06hj CATGGTTAAAAGTTCTAGCTGCAAATCCAGAG(+ATATAT)GATCCTGGTTCAATTCCCAGCACATACACGG NGG 10 8 17 8 24 0 100 5 10
    240606215523_341l CAGCTTACTGTTGCAGCTGCCTGAGCAGCCTG(+ATATAT)GATCCTGCCTGAGGTTAGACCAGAGACCTCC NGG 10 8 17 8 24 0 100 5 10
    240606215521_pe0x GAGGAGGAGGTCTCTGGTCTAACCTCAGGCAG(+ATATAT)GATCCAGGCTGCTCAGGCAGCTGCAACAGTA NGG 10 8 17 8 24 0 100 5 10
    240606215518_ttxi ACCCTAAAGTTTCTAGAAGGAGGCAGGGAAGG(+ATATAT)GTACCCCAGGCTCCCCATCCCCAGGGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606215515_03hs GGACTCAGCTCCCTGGGGATGGGGAGCCTGGG(+ATATAT)GTACCCTTCCCTGCCTCCTTCTAGAAACTTT NGG 10 8 17 8 24 0 100 5 10
    240606215514_03ci AAACAGCTTCATGTTTGCCCCATTTCTGTCAG(+ATATAT)GATCCCCCAACCATGGGGGGCACTTGCACAA NGG 10 8 17 8 24 0 100 5 10
    240606215510_48a0 TATTTTTGTGCAAGTGCCCCCCATGGTTGGGG(+ATATAT)GATCCTGACAGAAATGGGGCAAACATGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606215508_f8wt GCCCTTAGCATGACGGGTTCTGCCCTTTGTGG(+ATATAT)GATCCGGTAAGGAAACTGAGAGGTTCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240606215506_omdi TTGAAACAAGGAACCTCTCAGTTTCCTTACCG(+ATATAT)GATCCCACAAAGGGCAGAACCCGTCATGCTA NGG 10 8 17 8 24 0 100 5 10