Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606215343_qv5l TGACCATTTCTGTAATTGGTACTGTCTGGCAG(+ATATAT)GTACCATGTGTGGCCTGGCTGTCCTGGAACA NGG 10 8 17 8 24 0 100 5 10
    240606215341_z8ci TGCTGTGTTCCAGGACAGCCAGGCCACACATG(+ATATAT)GTACCTGCCAGACAGTACCAATTACAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240606215339_ensa GGATGGACACAGGTCCCCCAGGGCCTAGGATG(+ATATAT)GTACCTTGAGAAGACCAGCCTCATCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240606215338_ud2i GGGCCATGGATGATGAGGCTGGTCTTCTCAAG(+ATATAT)GTACCATCCTAGGCCCTGGGGGACCTGTGTC NGG 10 8 17 8 24 0 100 5 10
    240606215337_mppy AACTCAATTCTTGTTGGAAAAACAACAAAAAG(+ATATAT)GTACCTAATCTAGTACAGCACCCTCAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606215336_kfe7 CCTGGCTGGCTGAGGGTGCTGTACTAGATTAG(+ATATAT)GTACCTTTTTGTTGTTTTTCCAACAAGAATT NGG 10 8 17 8 24 0 100 5 10
    240606215334_zvfb TGTATGTAAAGTTCCAGGGATTTCAAAATCTG(+ATATAT)GTACCTAATGTGGCAAATGCACGGCTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240606215333_72q5 TAATCCTCACAGCCGTGCATTTGCCACATTAG(+ATATAT)GTACCAGATTTTGAAATCCCTGGAACTTTAC NGG 10 8 17 8 24 0 100 5 10
    240606215331_wj0s CATGTATTTATATGCACCACCAGAGTGCCTGG(+ATATAT)GATCCTGAAATCAGAAGACAGCATTAGGTCC NGG 10 8 17 8 24 0 100 5 10
    240606215330_22v4 TTCTAGGACCTAATGCTGTCTTCTGATTTCAG(+ATATAT)GATCCCAGGCACTCTGGTGGTGCATATAAAT NGG 10 8 17 8 24 0 100 5 10