Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606215252_vfkz CTTGATCCCAGGTGGATTCCCACCCTAACTGG(+ATATAT)GTACCAGGACTCTGAACAGGCCAAGATGGGG NGG 10 8 17 8 24 0 100 5 10
    240606215250_owv4 GAGTGCCCCATCTTGGCCTGTTCAGAGTCCTG(+ATATAT)GTACCCAGTTAGGGTGGGAATCCACCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606215248_vd3p GTGTCTCTTGGCACTGTCACCAAGTTGCCCAG(+ATATAT)GATCCTGGGGATGCTGTAATTTGGAAAATTG NGG 10 8 17 8 24 0 100 5 10
    240606215245_dyji GCCCTCAATTTTCCAAATTACAGCATCCCCAG(+ATATAT)GATCCTGGGCAACTTGGTGACAGTGCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606215243_uuc7 CAGCAGCCACTGGGAAGAAGAGGCACAACAAG(+ATATAT)GATCCATCCTCCCAGAGACCTTTCCAACTCC NGG 10 8 17 8 24 0 100 5 10
    240606215242_shgn TGGCAGGAGTTGGAAAGGTCTCTGGGAGGATG(+ATATAT)GATCCTTGTTGTGCCTCTTCTTCCCAGTGGC NGG 10 8 17 8 24 0 100 5 10
    240606215240_rf4q TCCTGGGCTTGGGCAGTGAGAGGAGCATCTAG(+ATATAT)GTACCTGTTGTGCCCCTAATAAAGTGTTGGA NGG 10 8 17 8 24 0 100 5 10
    240606215239_5lze TGTCATCCAACACTTTATTAGGGGCACAACAG(+ATATAT)GTACCTAGATGCTCCTCTCACTGCCCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606215237_tzta AATGAGTGCCTTCGCAGCCTGGTAGAAGAGAG(+ATATAT)GATCCGATTCGTCACCATCAGAATCCACTTT NGG 10 8 17 8 24 0 100 5 10
    240606215236_nahd TGGGGAAAGTGGATTCTGATGGTGACGAATCG(+ATATAT)GATCCTCTCTTCTACCAGGCTGCGAAGGCAC NGG 10 8 17 8 24 0 100 5 10